cd39 ax700 (R&D Systems)
Structured Review
Cd39 Ax700, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd39/Human+CD39%2FENTPD1+Alexa+Fluor%C2%AE+700-conjugated+Antibody/pm42822324-60-35-37
Average 94 stars, based on 1 article reviews
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Immunohistochemistry:Article Title: Selective deletion of ENTPD1/CD39 in macrophages exacerbates biliary fibrosis in a mouse model of sclerosing cholangitis Article Snippet: .. Primary antibodies were F4/80 (#MCA497; Bio-Rad, Hercules, CA, USA) and Staining:Article Title: Loss of survival factors and activation of inflammatory cascades in brain sympathetic centers in type 1 diabetic mice Article Snippet: .. Sections on slides were stained with Iba-1 (Wako, Osaka, Japan) for visualization of microglia/macrophages ( 50 ), other:Article Title: Negative feedback control of neuronal activity by microglia. Article Snippet: Membranes were then washed and probed with horseradish-peroxidase conjugated anti-mouse (GE), anti-rabbit IgG (GE), anti-rat IgG (Invitrogen) or anti-sheep IgG ( Jackson Immunoresearch, USA) secondary antibodies (all 1:10,000) for one hour at room temperature. Article Title: Negative feedback control of neuronal activity by microglia Article Snippet: 20-40 μg of tissue protein samples were separated on 4-12% Bolt Bis-tris precast denaturing gels or 10% NuPAGE Bis-tris precast denaturing gels (Invitrogen, USA) and transferred onto PVDF membranes. Western Blot:Article Title: Negative feedback control of neuronal activity by microglia. Article Snippet: TRAP: mouse monoclonal anti-GFP (clone 19F7 (cat# HtzGFP19F7) and clone 19C8 (cat# HtxGFP-19C8), Antibody & Bioresource Core Facility Memorial Sloan Kettering Cancer Center) FACS: CD39-A700 (clone 24DMS1, Cat#56-0391-82, ThermoFisher), CD73-PE (clone eBioTY/11.8, 12-0731-82, ThermoFisher) Validation Immunostaining antibodies: IBA1 (019-19741) CD11B (MCA711), P2RY12 (AS-55043A) are validated for immunostaining of microglia in mouse on the manufacturers website. .. Immunoblotting: Primary Antibodies: DARPP32, Novus, Cat#NB300-304, (1:1000); DARPP32-THR34, clone D27A4, Cell Signaling, Cat#12438, (1:1000); DARPP32-THR75, clone cc911, (1:1000) kindly provided by A Nairn and P Greengard (not commercially available); DARPP32, clone 6a, (1:5000) kindly provided by A Nairn and P Greengard (not commercially available); IL34, R&Dsystems, Cat#AF5195, (1:1000); GLUR1SER845, clone D10G5, Cell Signaling, Cat#8084, (1:1000); GLUR1, clone RH95, Millipore, Cat#MAB2263, (1:1000), DRD1, Abcam, Cat#ab20066, (1:1000); |
